Research Roundup – ASM Microbe 2022 Scientific Posters on Colibrí™

June 28, 2022

2nd

Welcome to Research Roundup, where COPAN will feature a comprehensive review and commentary on recent scientific publications covering a particular topic of interest. COPAN’s Research Roundup is authored by Dr. Susan Sharp, COPAN’s Scientific Director!

In this month’s edition, Dr. Sharp summarizes research posters regarding COPAN Automation from ASM 2022 . 

Scientific Review of ASM 2022 Posters

The 2022 American Society for Microbiology Microbe Conference was held this June in Washington, DC.  Members of the Microbiology community were thrilled to come together in person for this meeting for the first time since 2019.  In this Research Roundup, we highlight three posters that examined COPAN’s full laboratory automation which showcase the ability of the automation and software to select and process colonies for accurate identification by MALDI-TOF mass spectrometry (MS) and for antimicrobial susceptibility testing using the bioMerieux Vitek 2. 

Two posters examined accuracy for spotting MALDI plates using the Colibrí™ automated specimen workup instrument with colonies selected using WASPLab® full laboratory automation system .

Studies on Colibrí™ Accuracy for Spotting MALDI Plates using WASPLab® Full Laboratory Automation System

Study 1 – Evaluation of Colibrí™ MALDI-TOF Spotting

Evaluation of the Colibrí™ from Copan for automated MALDI-TOF spotting

E. Zarate, E. Pickering, C. Murphy, J. Morejon, K. Bryant
HNL Lab Medicine, Allentown PA, Kaiser Permanente, Portland, OR

In this two-site study, Heath Network Laboratories (Allentown, PA) and Kaiser Permanente (Portland, OR) colonies from routine culture plates of urines, wounds, blood cultures, and respiratory specimens were incubated in the WASPLab® and assigned pickpoints after 18 to 24 hours of incubation. The culture plates were then transferred to Colibrí™ for automated MALDI ID plate spotting and matrix application. Manual spotting was then performed using the same or ‘like’ colonies. A total of 242 bacterial organisms were compared.

Study Conclusion

This image has an empty alt attribute; its file name is Gram_Positive_Infographic_062822-1170x840.jpg

Of 87 Gram-positive organisms (49 Staphylococcus spp., 34 Streptococcus/Enterococcus spp., and one organism from four other different genera) 85/87 were successfully identified using the Colibrí™ for a concordance of 100%. 2 Colibrí™ spots (1-Staphyloccocus haemolyticus and 1-Gemella haemolysans) gave no identification; the S.haemolyticus was available for retesting and was successful on repeat with the Colibrí™. 154/155 Gram negative organisms were compared with a concordance of identification of 100%. One Colibrí™ spot was unsuccessful with a mucoid K. pneumoniae but was successful upon repeat testing.

Study 2 – Evaluation of Colibrí™ MALDI-TOF Target Plate Preparation

Evaluation of Automated MALDI-TOF Target Plate Preparation

My-Lien Pham*, Ken Van Horn, Utsav Pandey
Southern California Permanente Medical Group, Chino Hills, CA

Performed at Southern California Kaiser Permanente (Chino Hills, CA) 218 identifications of bacteria isolated from urine cultures were compared between Colibrí™ spotting and manual spotting.

Study Conclusion

Only two discrepancies were observed:

1) Streptococcus agalactiae (manual) / Proteus mirabilis (Colibrí™).
NOTE: This was believed to be an incorrect plate tested.

2) Raoultella ornithinolyticus (manual) / Enterobacter complex (Colibrí™).

The accuracy of identification between the two methods was 99.1%. No purity plates were examined in this study and repeat testing was not performed. 4.4% of organisms yielded no identification when tested by MALDI-TOF processed by Colibrí™ (4 Enterococcus faecalis, 3 Streptococcus agalactiae, 1 S. gallolyticus spp., 1 S. pyogenes and 1 E. coli); these isolates were also not retested, but by procedure, these isolates would be repeated with manual spotting and were not considered discrepant results.

Summary Conclusions of Studies on Colibrí™and MALDI-TOF

CONCLUSIONS

Colibrí™ provides automation to a previously manual process with MALDI-TOF target spotting.

A high level of concordance between manual and automated processing was observed.

Colibrí™ provides an opportunity to reallocate technologist time for more complicated task

Colibrí™ helps to standardize spotting and provides traceability from plating to organism identification.

Colibrí™ is an accurate method for preparation of MALDI-TOF target plates.

A Study Comparing AST testing using McFarland suspensions prepared by the Colibrí™ to those prepared Manually.

Study 3 – Automated Colibrí™ vs Manual Colony Picking and McFarland Preparation

Automated colony picking and McFarland preparation using the Copan Colibri™ instrument is equivalent to manual McFarland preparations for antimicrobial susceptibility testing using the Vitek 2 system

K. Bryant, Kaiser Permanente, Portland OR

In a third study, presented at ASM Microbe 2022 and performed at Kaiser Permanente (Portland, OR), Dr. Kendall Bryant used the Vitek 2 (V2) AST system to compare McFarland suspensions prepared by the Colibrí™ to those prepared manually.  106 organisms were compared in the study (56 GNR, 50 GPC) using V2 antibiotic panels. The organisms were comprised of clinical isolates and reference organisms from the CDC AR Isolate Bank. A total of 1,732 drug/bug combinations were tested.

There were 6 discrepant results in categorical interpretations:

  • 1 with gentamicin-R (manual) and -I (Colibrí™)
  • 2 with nitrofurantoin-I (manual) and -R (Colibrí™)
  • 1 with amoxicillin/clavulanate-I (manual) and -R (Colibrí™)
  • 1 with nitrofurantoin-S (manual) and -R (Colibrí™)
  • 1 with a CDC organism where the Colibrí™ agreed with the CDC result of S while the manual result was I.

This gave an overall categorical agreement of 99.75%.

There were 5 instances of > 1 dilution difference in MIC results between the two methods.

  • 1 with cefazolin and Klebsiella pneumoniae
  • 1 with ampicillin and Escherichia coli
  • 1 with erythromycin and Staphylococcus hominis
  • 2 with daptomycin and Staphylococcus aureus

The overall +/- 1 dilution correlation was 99.99%.

Study Conclusion

1.) The performance of the Colibrí™ is equivalent to manual methods for McFarland suspension preparation used for V2 susceptibility testing.

2.) There was 99.75% categorical agreement and 99.99% agreement in MIC values (within +/-1 dilution) when comparing the Colibrí™ and the manual method for AST preparations.

3.) The Colibrí™ provides an automated option for the highly repetitive task of Vitek 2 AST setup freeing technologists time to perform other tasks in the laboratory.

The Roundup: Conclusions on Findings from ASM 2022 Posters on Colibrí™

These three posters show the ability of Colibrí™ to accurately spot MALDI ID plates and accurately prepare antibiotic susceptibility suspensions for automated testing.  The use of Colibrí™ for MALDI plate spotting and AST preparation is an automated, labor-saving step that will allow technologists to perform other, more highly skilled duties in the laboratory.

More on Colibrí™

Fully Automated Specimen Workup Instrument Designed to Prepare MALDI-TOF ID Targets and Create Bacterial Suspensions for Antibiotic Susceptibility Testing*

Stay Informed

With tens of thousands of scientific papers published each year, it is hard to stay informed on the relevant research. Each month COPAN’s Research Roundup will feature a comprehensive review and commentary on recent scientific publications covering a particular topic by experts in the field to help keep you up to date on the latest research. Sign up today so you don’t miss the latest findings!

About Dr. Susan Sharp

Dr. Sharp has been a clinical microbiologist and active member of the American Society for Microbiology (ASM) for 30 years serving on various boards and committees.  She is a Diplomat of the American Board of Medical Microbiology (ABMM) and a Fellow in the American Academy of Microbiology.  Dr. Sharp served as the Chair of the ASM Public and Scientific Affairs Board’s Committee on Laboratory Practices from 2007 to 2015, as well as Chair of the Examination Development committee and Vice-Chair of the ABMM during 1999-2015.

Dr. Sharp has recently served as an Advisor to the CLSI Antimicrobial Susceptibility Testing Sub-Committee and she is a current member of the CLSI AST Resistance Working Group as well as the Methods Application and Interpretation Working Group.  She is also an active member of the Board of Scientific Councilors for the Office of Infectious Diseases of the Centers for Disease Control and Prevention.  Dr. Sharp is a Past-President of ASM and a former member of ASM’s Board of Directors. Dr. Sharp has given numerous lectures, seminars and workshops locally, nationally and internationally, and has numerous publications in the field of clinical microbiology.  Her most prominent area of interest has centered on cost-effective, clinically-relevant diagnostic microbiology.

About COPAN Diagnostics, Inc.

With a reputation for innovation, COPAN Diagnostics is part of COPAN Group, the leading manufacturer of collection and transport systems in the world.  COPAN’s collaborative approach to pre-analytics has resulted in Flocked Swabs, ESwab™, UTM Universal Transport Medium®, and laboratory automation, WASP® and WASPLab®.  COPAN carries a range of microbial sampling products, inoculation loops, and pipettes. For more information, visit www.copanusa.com

Previous Blog Posts