Evaluation of Two Newly Developed QIAsymphony SP Protocols for Efficient Isolation of Influenza Virus RNA from Diff. Respiratory Samples

Introduction:
Influenza is one of the most severe respiratory infections. The human population regularly experiences influenza pandemics. Novel influenza A (H1N1) virus, commonly known as swine flu, is a new influenza virus causing illness in humans. This new influenza virus was first detected in April 2009. Detection of influenza virus by real-time CR is sensitive and fast. The artus Infl./H1 LC/RG RT-PCR Kit contains an influenza A (H1N1) specific detection reagent, enabling additional detection of the 2009 pandemic H1N1 virus.
Sensitive detection of influenza RNA by real-time PCR requires purified RNA free inhibitors. Here, we report the suitability of the QIAsymphony SP for isolation of influenza RNA from different sample materials using the QIAsymphony Virus/Bacteria Mini Kit in combination with the Complex 200 protocol. In addition, this fully automated protocol is compared to a QIAsymphony protocol with manual off-board lysis.

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