Sinonasal Microbiome Sampling: A Comparison of Techniques
Categories: Bacteriology, Flocked Swabs
Publication Date: 06/09/2015
Conference or Journal: PLOS One
Author(s): Ahmed Bassiouni, Edward John Cleland, Alkis James Psaltis, Sarah Vreugde, Peter- John Wormald
The role of the sino-nasal microbiome in CRS remains unclear. We hypothesized that the
bacteria within mucosal-associated biofilms may be different from the more superficiallying,
free-floating bacteria in the sinuses and that this may impact on the microbiome results
obtained. This study investigates whether there is a significant difference in the microbiota
of a sinonasal mucosal tissue sample versus a swab sample.
We observed no significant difference between the microbiota of mucosal tissue and swab
samples. This suggests that less invasive swab samples are representative of the sinonasal
mucosa microbiome and can be used for future sinonasal microbiome studies.